The invention relates to a device for holding a pipette in a pipetting aid making it possible to achieve a gastight fit of the pipette into the pipetting aid without requiring the exertion of any pressure by the handler, in which spreaders (2) are cast in a rubber or plastic aperture bead on the pipetting aid and the relative positions of the spreaders (2) can be altered by tensioners (3) in such a way that the aperture bead can be widened for the insertion of the pipette.