| 摘要 |
A colloidal gold immunoassay method for detecting a substance to be examined wherein the reaction mixture contains metal salt(s) selected from the group consisting of sodium fluoride, potassium fluoride, lithium fluoride, sodium iodide, potassium iodide, lithium iodide, magnesium iodide, sodium bromide, potassium bromide, lithium bromide, magnesium bromide, lithium chloride, magnesium chloride, sodium nitrate, potassium nitrate, lithium nitrate, magnesium nitrate, sodium sulfate, potassium sulfate, lithium sulfate, magnesium sulfate, sodium formate, potassium formate, lithium formate, magnesium formate, sodium acetate, potassium acetate, lithium acetate, magnesium acetate and mixtures of two or more of the same which have been dissolved in the immunoreaction system. In this method, the metal salt contributes to the extremely distinct color development of the colloidal gold. In the case of a specimen at a concentration in the vicinity of the detection limit, in particular, the positivity can be more clearly distinguished from the negativity with the naked eye, which facilitates the judgement. |
