NOVEL SYSTEM FOR MULTILAYER GROWTH OF ANCHORAGE-DEPENDENT CELLS

摘要

Anchorage-dependent cells are grown on a novel substratum which is belived to increase the oxygenation of the cells and reduce the formation of free-radicals. The substratum consists of a perfluorocarbon reservoir which is coated with a layer of protein (e.g., collagen or gelatin) which has been perfluoroalkylated. The perfluoroalkylated protein forms a very stable surface to which anchorage-dependent cells can attach and grow. Using this system, mammalian cell cultures have been grown to densities exceeding 10<7> cells/cm<2>. The increased density is attributed to the formation of multiple cell layers which resemble tissue-like structures.