摘要 |
PURPOSE:To readily obtain diacetyl useful as an aromatic ingredient of fermented dairy products, etc., by adding one or more of Fe, Cu and Mo in the form of inorganic or organic salts to a culture medium containing citric acid and cultivating lactic acid bacteria. CONSTITUTION:Inorganic or organic salts of Fe, Cu and Mo are added to a culture medium consisting of a skimmilk or skimmilk powder solution containing about 1wt.% citric acid in solid matter so as to provide 0.01-10mM total amount of Fe ions, Cu ions and Mo ions. Streptococcus lactis subsp. diacetylactis which is a lactic acid bacterium having the ability to ferment citric acid is then inoculated into the resultant culture medium and fermented at about 30 deg.C for about 16hr to enhance the amount of produced diacetyl. |