| 摘要 |
PURPOSE: To obtain a new gene extracted out from the surgical nucleation cell specimen of human malignant glioma, coding the human small TIMP2 having a specific base sequence, and useful for determining the malignancy of human cerebral tumor, for diagnosing the risk of cancer, etc. CONSTITUTION: This new DNA has a base sequence of the formula and codes human small TIMP(Tissne Inhibitor Metaro Protease) 2. The gene is useful as a primer or probe for examining the malignancy of human cerebral tumor or for risk diagnosis, preliminary preventing treatment, progress observation, therapeutic policy determination and aftercare estimation about the tumor. The gene is obtained by separating mRNA from the surgical enucleation cell specimen of human malignant glioma by a conventional method, synthesizing cDNA from the mRNA, and subsequently cloning the obtained single strand cDNA as a template with PCR. |
