Trypsin (trypsinogen) may be produced in a filamentous fungus by transforming a filamentous fungus with a vector comprising a DNA sequence encoding protrypsin or a derivative thereof N-terminally fused to a DNA sequence encoding a signal peptide, culturing the transformed filamentous fungus in a suitable culture medium to produce trypsinogen and recovering trypsinogen and/or trypsin from the medium.
申请公布号
WO9700316(A1)
申请公布日期
1997.01.03
申请号
WO1996DK00253
申请日期
1996.06.10
申请人
NOVO NORDISK A/S;WOELDIKE, HELLE, FABRICIUS;KJELDSEN, THOMAS, BOERGLUM