| 摘要 |
<p>Mutations in the spoIII gene of B Subtilis abolish sporulation by preventing partition of a prespore chromosome into the small polar prespore compartment. The invention provides a Bacillus strain having a chromosome with two reporter genes each linked to a promoter and responsive to the action of σF during sporulation, one located inside and the other located outside a segment of the DNA that is trapped in a prespore compartment; and use of the strain in a method of determining whether an agent inhibits SpoIIIE function in Bacillus species.</p> |
