MODIFIED POLYPEPTIDE, DNA ENCODING THE SAME, TRANSFORMANT, AND MEDICINAL COMPOSITION CONTAINING THE POLYPEPTIDE

摘要

A polypeptide having the amino acid sequence wherein Asn at the 18-position of an amino acid sequence consisting of the amino acids at the 1- to 77-positions of the amino acid sequence of natural human MACIF active protein has been replaced by Gln. This polypeptide is produced and harvested by incubating cells transformed by an expression vector containing a DNA encoding the polypeptide. The modified polypeptide inhibits cytolysis by the complements of nuclear cells and the release of PGI2 and b-FGF by the complements from HUVEC. Also, it inhibits a hyperacute rejection in a model reflecting a hyperacute rejection of transplantation.