| 摘要 |
A multilayer analysis element for determination of total cholesterol is prepared having a light-transmissive water-impermeable support, at least one hydrophilic polymer layer on said support and a spreading layer on said hydrophilic polymer layer(s), and containing in one or more of the layers: (a) at least one enzyme having cholesterol esterase activity, (b) cholesterol oxidase, (c) peroxidase, (d) a color reagent composition, which in the presence of hydrogen peroxide and the peroxidase, produces a color change (e) at least one bile acid compound selected from the group consisting of bile acids, bile acid derivatives and salts of said acids and derivatives, and (f) an alkyl phenoxy polyethoxy ethanol containing in the alkyl group 1 to 20, preferably 7 to 10 carbon atoms, and a polyoxyethylene chain composed of at least 16, preferably 30 to 60 oxyethylene units.
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