| 摘要 |
The present invention provides novel, artificial, restriction endonucleases comprising the DNA binding motif of the transcription factor Spl and the C-terminal DNA cleavage domain of FoKI restriction endonuclease. The new restriction endonucleases are designated herein as "splase enzyme" and "Hsplase enzyme". The restriction endonuclease of the present invention recognizes a 10 base nucleotide sequence and thus, since the probability of these particular 10 base sequences occurring frequently in a DNA sample is low, the restriction endonucleases only rarely cleave the DNA sample. Thus, the Splase enzyme and Hsplase enzyme are particularly useful cutting DNA into large fragments rather than into a myriad of fragments as is common with conventional restriction endonucleases. The invention also relates to artificial fusion genes which encode the artificial restriction endonucleases including the artificial restriction endonucleases Splase enzyme and Hsplase enzyme. The DNA molecule, that is the genes that encode the Splase enzyme and Hsplase enzyme are designated herein as the "Splase gene" and the "HSplase gene", respectively. The invention also relates to vectors and cells that contain the artificial genes. |
