PROCESS FOR PREPARING LIMULUS AMOEBOCYTE LYSATE

摘要

A process for producing a limulus amoebocyte lysate which specifically reacts with endotoxin, which comprises bringing a limulus amoebocyte lysate into contact with an insoluble, immobilized (1->3)- beta -D-glucoside structure to remove a (1->3)- beta -D-glucan-sensitive factor (G factor) contained in the lysate. Since the resulting lysate is substantially free from the (1->3)- beta -D-glucan-sensitive G factor, it specifically reacts with an endotoxin present in biological specimens such as blood, urine or spinal fluid to thereby allow the endotoxin to be assayed with a high sensitivity, thus contributing particularly to clinical examination.