IMMUNOLOGICAL DETERMINATIONS

摘要

A method of effecting an immunological determination of an analyte in a fluid sample, the method comprises the steps of (i) providing a column of a solid support material having a first binding agent which is covalently linked to the solid support material and which is capable of forming an immunological complex with said analyte, (ii) introducing the fluid sample onto the column to allow analyte (if present) therein to form an immunological complex with said first binding agent, (iii) providing a label bound to the immunological complex formed under (ii), and (iv) using the immobilised label in situ on the support to determine the analyte.