摘要 |
<p>An oligonucleotide having a terminal 5'-OH group is reacted with a phosphitylating agent or phosphorylating agent and then with a phosphate or pyrophosphate and an oligonucleotide having a terminal 5'-di- or triphosphate group is recovered. The resulting oligonucleotide having a terminal 5'-di- or triphosphate group may be capped and used for the study of influenza virus transcription.</p> |