发明名称 |
Modified adeno-associated virus vector capable of expression from a novel promoter |
摘要 |
Described herein are constructions of recombinant DNA comprising modified adeno-associated virus (AAV) DNA sequences capable of functioning as a eukaryotic expression vector for expressing foreign DNA sequences using a novel transcription promoter comprising the termini of AAV DNA. It is shown that expression of a test reporter gene can be obtained from this vector in mammalian cells. It is further shown that this combination of vector and promoter can be used to introduce and express a human gene and correct a genetic defect in human cells resulting from malfunction of the mutant endogenous gene. Further, the vector can be used to correct the genetic defect by expressing a modified version of the human gene consisting of a fusion of part of the said gene and a synthetic sequence contained in the vector.
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申请公布号 |
US5587308(A) |
申请公布日期 |
1996.12.24 |
申请号 |
US19920891962 |
申请日期 |
1992.06.02 |
申请人 |
THE UNITED STATES OF AMERICA AS REPRESENTED BY THE DEPARTMENT OF HEALTH & HUMAN SERVICES |
发明人 |
CARTER, BARRIE J.;FLOTTE, TERENCE;AFIONE, SANDRA;SOLOW, RIKKI |
分类号 |
A61K38/00;A61K48/00;C07K14/47;C12N15/12;C12N15/864;(IPC1-7):C12N5/10;C12N15/86;C07H21/04 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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