Thermostable polymerase specific antibody-containing DNA amplification composition and kit

摘要

Antibodies which are specific to a thermostable DNA polymerase can be used to reduce or eliminate the formation of non-specific products in polymerase chain reaction methods. These antibodies and other temperature sensitive inhibitors are effective to inhibit DNA polymerase enzymatic activity at a certain temperature T1 which is generally below about 85 DEG C. The inhibitors are irreversibly inactivated at temperature T2 which is generally above about 40 DEG C. T2 is also greater than T1. Such inhibitors can be supplied individually or in admixture with the DNA polymerase in a diagnostic test kit suitable for PCR.