| 摘要 |
Metal binding polypeptides which include an amino acid sequence coding for a variable region of a monoclonal antibody which immunoreacts with a lead cation and nucleotides which include a nucleic acid sequence coding for the variable region are provided. The invention is also directed to fusion proteins which include the monoclonal antibody heavy chain variable region and a phage coat protein or portion thereof. The invention also provides bacteriophages which include the fusion protein in their coat. In addition, methods for detecting, removing, adding, or neutralizing lead cations in biological or inanimate systems through the use of the lead binding polypeptides are provided. A method of forming a hybridoma which produces monoclonal antibodies capable of immunoreacting with a metallic cation is also provided. |
