CATIONIC LIPID:DNA COMPLEXES FOR GENE TARGETING

摘要

The invention herein describes pharmaceutical compositions and methods for targeted delivery of functional genes into cells and tissues in vivo. The invention discloses DNA:lipid complexes, methods of making such complexes and methods of using such complexes for facilitating the targeted delivery and entry of recombinant expression constructs into cells and tissues in vivo, and particularly delivery of such recombinant expression constructs into cells and tissues in vivo, and particularly delivery of such recombinant expression constructs by intravenous, intraperitoneal or direct injection. The delivery vehicle for the targeting of the recombinant construct encoding the gene of interest, is composed of a mixture of a cationic imidazolinium lipid of formula (I) wherein R and R1 mean a C11-29 aliphatic hydrocarbyl group, together with neutral lipids (e.g. DOPE, cholesterol).