发明名称 |
CHONDROITINASE I AND CHONDROITINASE II PRODUCING MUTANTS OF P. VULGARIS |
摘要 |
Mutant Proteus vulgaris strains are provided that, when grown in the absence of an exogenous chondroitinase I and II inducer, produce P. vulgaris chondroitinase I and chondroitinase II proteins. The mutants typically produce chondroitinase I and II proteins in the absence of exogenous inducers and in amounts in excess of those produced by wild-type P. vulgaris strains induced with such inducers. Two classes of such mutants, Classes 1 and 2, are disclosed. Class 1 and class 2 mutants differ in the relative amounts of chondroitinases I and II produced when cells are grown in casamino acids - supplemented minimal medium. Additional phenotypic variants that release chondroitinase I protein into the culture medium are provided as well. Also contemplated is a method for producing P. vulgaris chondroitinase I and II proteins. The mutant cells described above are cultured in the absence of a conventional exogenous chondroitinase I and II inducer, after which the cells are harvested and chondroitinase I and II are recovered from the harvested cells. A method for in situ detection of chondroitinase I and II production by bacterial colonies is also provided. |
申请公布号 |
WO9640897(A2) |
申请公布日期 |
1996.12.19 |
申请号 |
WO1996US08593 |
申请日期 |
1996.06.04 |
申请人 |
AMERICAN CYANAMID COMPANY |
发明人 |
LOTVIN, JASON, ARNOLD;KHANDKE, KIRAN, MANOHAR;RUPPEN, MARK, EDWARD |
分类号 |
C12N15/09;C12N1/21;C12N9/88;C12Q1/527;C12R1/19;C12R1/37 |
主分类号 |
C12N15/09 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|