| 摘要 |
A portable apparatus for detecting the presence of methylxanthine chemical species such as caffeine or theophylline in a solution such as a beverage comprises a first portion comprising an effective concentration of at least one phosphodiesterase enzyme, a second portion comprising an effective concentration of cyclic AMP, and means for indicating the inhibition of the degradation of cyclic AMP by phosphodiesterase due to the presence of at least one methylxanthine compound. A method for determining the presence of methylxanthine chemical species in a solution such as a beverage comprises contacting at least one test portion of the solution with effective concentrations of at least one phosphodiesterase enzyme and cyclic AMP, and therafter contacting the test portion with means for indicating the inhibition of the degradation of cyclic AMP by phosphodiesterase due to the presence of at least one methylxanthine compound. The apparatus and method are advantageous in that they provide a simple and effective means of determining whether methylxanthine chemical species such as caffeine or theophylline are present in solutions such as coffee, tea and other beverages. |
