| 摘要 |
<p>This invention relates to peptide ligand discovery and is particularly directed to a method for the discovery of agonists for membrane bound receptors. The inventive detection system involves the use of a 'tethered' ligand for probing receptor binding. The general detection system comprises: a membrane, a membrane bound receptor, and a chimeric ligand presenting molecule. This chimeric protein forms the tethered ligand and in turn comprises: a membrane domain, a linker domain, a ligand domain, and a cleavable terminal domain. The 'ligands' of the system are exposed by the addition of a specific peptidase that cleaves at the designated sequence. The sequence of the ligand that produces signal as a result of the interaction between the agonist and receptor can then be isolated using sib selection.</p> |
