| 摘要 |
Modified pyrimidine bases and nucleotides containing a linker arm and a detectable reporter group for nucleic acid labeling. The modified nucleotides have structure (1), wherein: -R1 is -H or -OH; -R2 and -R3 are independently -H, -OH, monophosphate, diphosphate, triphosphate, thio analogs of mono-, di-, or triphosphates, -O- attached to a reactive phosphorous-containing group or -O- protected by a blocking group; -A and -B are independently -H, an electron donating group, an electron withdrawing group, C1-C4 alkyl, halogen, nitro, -COOH, amino, substituted amino, cyano, -CONH, -CSNH, -COOR4, (a), (b), -CSOR4, -COSR4, -SR4, -COR4, -CH2NHR4, -R4C=CR5H or -CC-R4 where R4 and R5 are independently H, an electron donating group, an electron withdrawing group, C1-C4 alkyl, halogen, nitro, COOH, amino, substituted amino, cyano, CONH or CSNH; -X- is -NH-, -NHNH-, -O-, -S-, (a) or -CH2-; Rep is a detectable reporter group; an d -L- is -(CH2)n-(T)q-(CH2)mZ1 or -(CH2)n(T)q-(CH2)m-Z2-(CH2)pZ1 where n, m and p are each independently an integer from 1 to 20 and q is 1 or 2, -T- is -O-, -S-, (a), (c), (d), (e), (f), -NH- or a heterocyclic structure containing one or more Group Va or VIa elements as a ring atom, Z1 is a covalent bond, -NH-, -CO-, -O-, -S- or (a) and -Z2- is -NH-, -NHCO-, -NHCS-, -CONH-, -CSNH-, -O-, -S- or (a).
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