Verfahren zum ortsaufgelösten immunhistochemischen Nachweis von Steroidrezeptoren
摘要
The invention concerns a method for the locally-resolved immune-histochemical detection of steroid receptors in tissue containing target cells, the method comprising the following steps: A tissue sample containing target cells is treated with an aqueous formaldehyde solution with a pH of less than about 6.0, a non-covalently bound ligand on the steroid receptor being set free from the steroid receptor and covalently bound in the molecular vicinity of a sterically open bonding point. The tissue sample is subsequently dehydrated and embedded in a standard embedding compound. The embedded tissue sample is sliced to produce a sequence of slices. At least some of the slices are rehydrated and incubated with a ligand-specific antibody, the ligand-specific antibodies adding to the ligands bound at sterically open bonding points. The added ligand-specific antibodies are then rendered visible by means of a second antibody/enzyme conjugate pair and by its reaction with a substrate to give an insoluble product.
申请公布号
DE19516954(C1)
申请公布日期
1996.12.19
申请号
DE19951016954
申请日期
1995.05.12
申请人
MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V., 80539 MUENCHEN, DE
发明人
JUNGBLUT, PETER W., PROF. DR., 31535 NEUSTADT-BUEREN, DE;SIERRALTA, WALTER D., DR., 30625 HANNOVER, DE