发明名称 |
IN VITRO PACKAGING OF ADENO-ASSOCIATED VIRUS DNA |
摘要 |
A method for in vitro packaging of adeno-associated viral particles is described. The procedure involves the preparation of cell-free extracts containing all the essential components for packaging. Homogeneous purified substrate DNA for packaging may be prepared separately. The in vitro packaged AAV particles are useful in transduction of mammalian cells and for gene therapy in animals. In one described method, the DNA packaged into AAV particles is not limited by the size constraints characteristic of in vivo packaged AAV particles.
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申请公布号 |
WO9640270(A1) |
申请公布日期 |
1996.12.19 |
申请号 |
WO1996US09263 |
申请日期 |
1996.06.05 |
申请人 |
RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YOR |
发明人 |
ZHOU, XIAOHUAI;MUZYCZKA, NICHOLAS;ZOLOTUKHIN, SERGEI;NI, TIEHUA |
分类号 |
C12N15/09;A61K35/76;A61K48/00;C12N5/10;C12N7/00;C12N7/01;C12N15/864;(IPC1-7):A61K48/00;A61K49/00;C12N15/10;C12N15/86 |
主分类号 |
C12N15/09 |
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