MONOCLONAL ANTIBODY FRAGMENT TO HUMAN OVARIAN CANCERS

摘要

The biodistribution and pharmacokinetics of intact and F(ab')2 fragments of radiolabeled (<125>I) monoclonal antibody (Mab) MX-35 and a control mAB were compared in nude mice bearing xenografts of OVCAR-3 human epithelial ovarian cancer. The highest tumor to blood ratio was 12.8 for the F(ab')2 fragment and 1.9 for intact Mab MX-35. Maximum tumor to normal tissue ratios were 64 for fragment and 12.3 for intact antibody. The maximum percent injected dose per gm (%IDg<-1>) of Mab in tumor was 10.4 % for F(ab')2 fragments and 1.6 % for intact antibody when administered intravenously and 8.2 % for F(ab')2 fragments and 2.3 % for intact MX-35 when injected intraperitoneally. The finding of higher percentage ID g<-1> for the fragments is unexpected and is in contrast to other published studies.