Methods and kits for determining pre-amplification levels of a nucleic acid target sequence from post-amplification levels of product

摘要

Methods and kits for relating initial amounts of target nucleic acids present in a sample to target-specific amplification products. It has been discovered that the transcription-mediated amplification system is capable of producing a quantitative relationship between target input and target-specific output. Further, the present invention relates to methods for carefully controlling this relationship resulting in an unexpectedly high degree of reproducibility. Also described are useful methods for extending the dynamic range of transcription-based amplification systems. <IMAGE> <IMAGE> <IMAGE> <IMAGE> <IMAGE> <IMAGE>