MANUFACTURE OF NEW MICROBE, L-ALPHA-AMINO ACID, AND CULTURE METHOD FOR MUTANT STOCK AND VARIANT OF NEW MICROBE, OBTAINING METHOD FOR GENE WHICH CODES CARBAMOYLASE AND/OR HYDANTOINASE AND/OR HYDANTOIN-RACEMASE, AND INSERTING METHODOF GENE WHICH CODES CARBAMOYLASE AND/OR HYDANTOINASE AND/OR HYDANTOIN-RACEMASE INTO MICROBE OR CELL

摘要

DSM 9771 is a mutant of DSM 7330 which was obtained under selective pressure. Its enzymatic activity is higher by a factor of 2.3 than that of its parent organism. In the presence of an inducer, this activity may be farther increased by a factor of 2.7. The reaction catalyzed by this microorganism or enzymes therefrom is the enantioselective conversion of a D-5-monosubstituted hydantoin or an L-5-monosubstituted hydantoin or a D-N-carbamoyl amino acid or an L-N-carbamoyl amino acid to a corresponding L- alpha -amino acid.