TRANSCRIPTIONAL ABNORMALITY OF INTRACELLULAR HSP70MRNA UNDER SUSTAINED STRESS LOAD CONTAINING HUMAN ACUTENESS AND CHRONICITY AND ITS APPLICATION (APPLICATION OF BOTH TRANSCRIPTIONAL EXPRESSION OF NEW HUMAN INTRACELLULAR SHSP70MRNA AND TRANSCRIPTIONAL ABNORMALITY OF HSP70 MRNA)

摘要

PURPOSE: To obtain a new probe, having the complementation of a DNA region of an mRNA in a heat shock protein located at a specific position on a human chromosome and a transcriptional region to an mRNA and useful for detecting the transcriptional abnormality of mRNA due to a human sustained stress load. CONSTITUTION: The new DNA and RNA probe contain nucleic acid sequence Nos. -90 to 810 represented by the formula of a substantial single-stranded nucleic acid sequence capable of selectively hybridizing a DNA region of a messenger RNA (HSP70mRNA) in a heat shock protein of 70kDa located at the 6p21.3-22nd and 14q22-24th and 21st positions on a human chromosome and a transcriptional region to a messenger RNA. The DNA and RNA probe are useful for detection, etc., of the transcriptional abnormality of an intracellular HSP70 mRNA under a sustained stress load containing the human acuteness and chronicity. The probe is obtained by separating a lymphocytic fraction from human peripheral blood, isolating an mRNA, then preparing a cDNA library with the isolated mRNA and cloning the cDNA library according to the suitable primer polymerase chain reaction(PCR).