发明名称 |
METHOD OF MAKING HERPES SIMPLEX TYPE 1 MUTANTS AND MUTANTS SO PRODUCED |
摘要 |
A method for introducing a mutation into a desired site in Herpes Simplex Virus Type 1 uses a set of starting vectors, where each starting vector has a fragment of the substantially complete HSV-1 genome and also DNA which overlaps with a sequential genomic fragment contained in other starting vectors, so that upon co-transfection of a host cell, replication of viral DNA, and recombination, a mutated replicable virus is formed. The starting vector containing a gene which is to be mutated is replaced by replacement vectors. The replacement vectors contain a copy of the mutated gene and overlapping DNA, and genomic DNA which was present in the starting vector. A host cell is transformed with the replacement vectors and the remaining starting vectors under conditions allowing replication of viral DNA and recombination to form a replicating mutated virus. In preferred embodiments, the protease gene is mutated.
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申请公布号 |
CA2222686(A1) |
申请公布日期 |
1996.12.05 |
申请号 |
CA19962222686 |
申请日期 |
1996.05.28 |
申请人 |
MERCK & CO., INC.;MERCK & CO., INC. |
发明人 |
SHAFER, JULES A.;REGISTER, R. BRUCE |
分类号 |
C12N9/50;C12N15/10;(IPC1-7):C12N7/01;C12N15/57;C12N15/86 |
主分类号 |
C12N9/50 |
代理机构 |
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主权项 |
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地址 |
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