Nucleic acid primers for amplification of a mycobacteria rna template

摘要

Disclosed is a pair of primers to amplify a homologous region of the 16S rRNA of Mycobacteria. The primer pair performs better than a number of other primer pairs in a transcription-based amplification system. The amplified RNA can be detected with known probes for Mycobacteria. The primers, methods and kits are especially useful as an aid in the diagnosis of M. tuberculosis.