Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers

摘要

The present invention provides admixtures and methods for PCR amplification of a target nucleic acid in which amplification efficiency is increased by including phosphorothioated oligonucleotide primers and an exonuclease in the PCR reaction mix. Kite for amplification of a target nucleic acid are also provided.