Method and an apparatus for preparing tissue repair promoting substances

摘要

A method for isolating tissue repair promoting substances from human or animal blood, which method comprises collecting the human or animal blood from a single human or animal individual in a first container of a container system comprising at least first and second interconnected containers; centrifuging the container system containing the blood so as to separate the blood in various fractions including a plasma fraction; transferring at least part of the plasma fraction to the second container of the container system; subjecting the plasma fraction in the second container to a low temperature so as to obtain a precipitate comprising tissue repair promoting substances; concentrating the precipitate in the second container so as to obtain a first fraction comprising a major part of the non-precipitated material, and a second fraction comprising at least the major part of the precipitate and a minor part of the non-precipitated material; and separating the second fraction comprising the tissue repair promoting substances from the first fraction within the second container; and optionally heating the second fraction within one of the containers of the container system so as to dissolve the isolated precipitated tissue repair promoting substances. Fractions comprising tissue repair promoting substances derived from blood cells may also be isolated within the container system, and optionally combined with the second fraction comprising tissue repair promoting substances derived from blood plasma.