发明名称 |
Chemiluminescent dialkyl-substituted 1,2-dioxetane compounds, methods of synthesis and use |
摘要 |
A chemiluminescent assay method and compositions are described which use a dialkyl-substituted dioxetane which is deprotected to trigger a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with two nonspirofused alkyl groups which can be triggered by a reagent to generate light are disclosed. Dialkyl-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.
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申请公布号 |
US5578253(A) |
申请公布日期 |
1996.11.26 |
申请号 |
US19940344124 |
申请日期 |
1994.11.23 |
申请人 |
LUMIGEN, INC. |
发明人 |
SCHAAP, ARTHUR P.;AKHAVAN-TAFTI, HASHEM |
分类号 |
G01N21/78;C07C43/178;C07C69/017;C07C69/12;C07C69/22;C07C69/78;C07D321/00;C07F7/18;C07F9/12;C07F9/655;C09K11/07;C12Q1/34;G01N33/533;G01N33/58;(IPC1-7):C09K3/00 |
主分类号 |
G01N21/78 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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