| 摘要 |
PURPOSE: To efficiently obtain the subject enzyme by culturing Escherichia coli containing both a vector having a gene encoding DnaJ as a heat shock protein and a vector having a gene encoding transglutaminase. CONSTITUTION: A genome DNA of Escherichia coli is used as a template and a DNA primer comprising a part of a gene encoding DnaJ as a heat shock protein is used. The gene encoding the DnaJ as the heat shock protein is amplified by PCR method, cloned and incorporated into a manifestation vector to prepare a recombinant DNA. A gene encoding a transglutaminase derived from a fish such as Pagrus major is cloned, incorporated into a manifestation vector to prepare a recombinant DNA. Then Escherichia coli is transduced with the manifestation vectors containing these genes, transformed and cultured and a recombinant DNA is manifested in an overproduced state of the DnaJ as the heat shock protein to give the objective transglutaminase. |
