发明名称 |
Fremgangsmåte for fremstilling av et is-kjernedannende protein, plasmider og bakteriestammer |
摘要 |
Additives for promoting ice nucleation in ingestible biological materials at sub-zero temperatures comprising a protein capable of promoting said ice nucleation carried by GRAS microorganisms transformed by a vector carrying one or more genes coding for the expression of said protein or by fractions of said microorganisms. Said vector is a plasmid comprising in the correct reading frame a cloning vector and DNA coding for said protein capable of promoting ice-nucleation and in the case of a yeast GRAS microorganism substantially the complete yeast 2 micron plasmid, at least one yeast selectable auxotrophic marker and the dLEU2 allele of the LEU2 gene and, in the case of a Lactococcal GRAS microorganism, additionally a non-inducible erythromycin resistance gene and a lactococcal plasmid promoter and origin of replication. |
申请公布号 |
NO180181(B) |
申请公布日期 |
1996.11.25 |
申请号 |
NO19900004532 |
申请日期 |
1990.10.19 |
申请人 |
SOCIETE DES PRODUITS NESTLE SA |
发明人 |
HOTTINGER, HERBERT;NIEDERBERGER, PETER;PRIDMORE, DAVID;STAEGER-ROOSE, URSULA |
分类号 |
A23B4/08;A21D6/00;A23G9/32;A23G9/44;A23G9/52;A23L3/3571;A23L3/37;A23L3/375;A23L29/00;A61K35/14;C07K14/21;C12N1/19;C12N1/20;C12N1/21;C12N15/09;C12N15/74;C12N15/81;C12P21/00;C12P21/02;C12R1/01;C12R1/19;C12R1/225;C12R1/38;C12R1/865 |
主分类号 |
A23B4/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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