发明名称 |
Amplifikationsverfahren |
摘要 |
A method for the amplification of a target nucleotide sequence using a first primer comprising 1) a target binding nucleotide moeity which is substantially complementary to the desired portion of the target nucleotide sequence; and 2) a polynucleotide tail; the first primer being such that whilst an extension product thereof may itself serve as a template for primer extension to form an amplification primer extension product, formation of an amplification primer extension product comprising a sequence complementary to the polynucleotide tail is inhibited. |
申请公布号 |
DE69028891(D1) |
申请公布日期 |
1996.11.21 |
申请号 |
DE1990628891 |
申请日期 |
1990.08.30 |
申请人 |
ZENECA LTD., LONDON, GB |
发明人 |
NEWTON, CLIVE ROBERT, WINCHAM, NEAR NORTHWICH, CHESHIRE, GB |
分类号 |
C12N15/09;C07H21/00;C12Q1/68 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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