发明名称 |
IN VIVO SELECTION OF RNA-BINDING PEPTIDES |
摘要 |
The invention provides methods and kits suitable for large-scale in vivo screening of polypeptides for specific binding affinity to a selected RNA recognition sequence. Polypeptide(s) are screened as fusion proteins with a polypeptide having antitermination activity. Polypeptides having binding activity are identified by their capacity to activate transcription of a reporter gene via binding of the polypeptide being screened to the selected RNA recognition sequence. The invention also provides methods of screening RNA molecules for binding to a selected polypeptide.
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申请公布号 |
WO9636692(A1) |
申请公布日期 |
1996.11.21 |
申请号 |
WO1996US06513 |
申请日期 |
1996.05.08 |
申请人 |
THE REGENTS OF THE UNIVERSITY OF CALIFORNIA |
发明人 |
HARADA, KAZUO;MARTIN, SHELLEY, S.;FRANKEL, ALAN |
分类号 |
C12N15/09;C12N1/21;C12N15/10;C12Q1/68;(IPC1-7):C12N1/20 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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