Recombinant chimeric HIV-1 Gag-Env fusion proteins

摘要

Disclosed is the construction, expression, and purification of chimeric human immunodeficiency virus type 1 (HIV-1) Gag-Env fusion proteins. Gag-Env chimeras were generated by fusing the amino terminus (amino acids 512-611) of the Env protein to the carboxyl terminus of the Gag protein (either amino acids 121-406 or 308-406). These proteins were overexpressed in Escherichia coli, purified, and their immunologic properties ascertained. Both chimeric proteins displayed immunoreactivity towards antisera obtained from HIV-1 seropositive patients. These HIV-1 Gag-Env fusion proteins should provide useful antigens for the detection of HIV-1-specific antibodies.