| 摘要 |
PURPOSE: To obtain a safe preparation containing thrombomodulin as an active ingredient, acting on bone cells, effective for the absorption bone diseases such as hypercalciuria caused by osteoporosis, myeloma and lymphoma, and little in side effects. CONSTITUTION: This preparation for preventing and treating the absorption bone diseases contains thrombomodulin (hereinafter referred to TM) as an active ingredient. The TM is preferably a soluble TM originated from a natural human urine or a human soluble TM synthesized by a genetic recombination method. The objective preparation is preferably administered at a daily does of 0.1-100μmg/kg as the amount of the TM protein. The preparation can continuously be administered by retaining the preparation in a living body in the form of an imbedding medicine or an osmotic pressure pump in which the preparation is sealed. The human urine-originated soluble TM is obtained e.g. by filtering a raw urine with acrylic fibers to adsorb off urokinase in the urine on the fibers, desalting and concentrating the passed urine with an ultrafiltration membrane, and subsequently successively purifying the product with a DEAE cellulose column, a DIP-thrombine-agaro-chromatography, etc.
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