METHOD FOR ACTIVATING A REVERSIBLY INACTIVATED IMMOBILIZED ENZYME BY RELEASE FROM AN IMMOBILISING MOIETY AND ITS USE IN NUCLEIC ACID AMPLIFICATION REACTIONS

摘要

The invention provides a method of activating a reversibly inactivated enzyme or catalytic fragment or mutant thereof, wherein the enzyme is inactivated by attachment to an immobilizing moiety and is activated by release from said moiety, particularly heat-activated release and activation of enzymes, particularly for use in nucleic acid amplification techniques, the immobilized enzymes and kits for performing methods of the invention.