| 摘要 |
<p>A method of increasing oligonucleotide selectivity in recognition of nucleic acids is disclosed, the method including the steps of disposing in aqueous solution a first oligomer having high selectivity for a target polynucleotide and a second oligomer having high affinity for an adjacent segment of the target polynucleotide. The oligomers include first and second reactive groups capable of spontaneously and irreversibly forming stable covalent bonds between the oligomers in absence of added chemical reagents or enzymes. A method for hybridizing nucleic acids includes the steps of reversibly binding a first oligomer to a target oligo- or polynucleotide including base units complementary to base units of the oligonucleotide, reversibly binding a second oligomer to the target oligo- or polynucleotide including base units complementary to base units of the oligonucleotide adjacent to the first oligomer, and wherein one of the oligomers includes a nucleotide having a first reactive group proximate to a nucleotide of the other oligomer which includes a second reactive group capable of spontaneously forming a covalent bond with the first reactive group. The method further includes the steps of irreversibly covalently joining the oligomers together through the first and the second reactive groups having been brought in proximity to each other upon binding of the oligonucleotides on the target polynucleotide in the absence of added reagent or enzyme, dissociating the joined first and second oligomers from the target polynucleotide, and removing the target polynucleotide.</p> |
