| 摘要 |
PURPOSE: To culture the seed of a marine microalga for the production of docosahexaenoic acid, etc., in shortened induction period under a condition to keep a carbon source in the culture medium at the end of culture at a specific concentration ratio based on the initial carbon concentration. CONSTITUTION: An alga belonging to marine microalgae and capable of producing docosahexaenoic acid (e.g. Cryptheconidium cohnii ATCC 30021) is subjected to seed culture using at least one kind of hydrolyzed glucose, galactose, lactose, etc., as a carbon source under a condition to keep the carbon source in the culture medium at the end of culture at a concentration corresponding to 0.3-3.1 times the initial carbon source concentration of the main fermentation process. The seed of the culture liquid having a carbon source concentration corresponding to 0.3-1.3 times the initial carbon source concentration of the production medium for main fermentation is transferred to the production medium and cultured. The culture product is recovered from the cultured algal cell to obtain docosahexaenoic acid having cholesterol level lowering action, blood coagulation inhibiting action, learning function improving action, etc. |
