MONOCLONAL ANTIBODIES TO GLYCOPROTEIN P, THEIR PREPARING AND USE, CELLS PRODUCING THEREOF, IMMUNOLOGICAL DIAGNOSTIC TEST BASED ON THEREOF, THERAPEUTIC COMPOSITION

摘要

FIELD: immunology. SUBSTANCE: invention relates to monoclonal antibodies recognizing extracellular human glycoprotein P epitope that involves specific epitope-forming amino acid sequence, preferably located in the fourth extracellular loop of human glycoprotein P and, especially, containing in peptide composition corresponding to the Sequence 1. Preferable antibodies bind human glycoprotein P specifically and at high efficiency and can be used for detection of human "MYA"-cells presented at very low part of cell population and producing human glycoprotein P at very small amount. Indicated antibodies can be the whole antibodies or their antigen-binding fragment and can be prepared by hybridoma technique or recombinant DNA. Monoclonal antibodies are useful for detection and purification of cell producing human glycoprotein P, in part, when they present in heterogeneous cell population and also for testing multiple resistance to antibiotics in cells, for example, tumor cells. EFFECT: improved method of preparing, enhanced effectiveness of test.