发明名称 |
METHODE DE COUPLAGE SPECIFIQUE DE LA COIFFE DE L'EXTREMITE 5' D'UN FRAGMENT D'ARNM ET PREPARATION D'ARNM ET D'ADNC COMPLET |
摘要 |
The present invention relates to a method for the specific coupling of the cup of the extremity 5' of a eucaryotic mRNA fragment by means of a molecule functionalized by an amine function. The present invention also relates to a method for isolating the extremity 5' of mRNA and a method for the preparation of the single strand cDNA extremity complementary of the extremity 5' of mRNA and of double strand cDNA corresponding to the extremity 5' of mRNA. The present invention also discloses a method for isolating full length cDNA corresponding to the totality of mRNA.
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申请公布号 |
FR2733762(A1) |
申请公布日期 |
1996.11.08 |
申请号 |
FR19950009467 |
申请日期 |
1995.08.03 |
申请人 |
GENSET |
发明人 |
NICOLAEVNA MERENKOVA IRENA;DUMAS MILNE EDWARDS JEAN BAPTISTE |
分类号 |
C12N15/09;C07H21/02;C12N15/10;(IPC1-7):C12N15/10;C12N15/11;G01N33/53 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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