发明名称 |
Expression of tetanus toxin fragment C in yeast |
摘要 |
Expression of tetanus toxin fragment C is accomplished employing a DNA coding sequence having a (G+C)-content that has been increased in the region from nucleotide 410 to the 3' end of the coding sequence relative to the wild-type DNA sequence. This allows the production of complete mRNA transcripts. Typically the (G+C)-content is increased in the following regions: (i) nucleotides 510-710, (ii) nucleotides 650-850, (iii) nucleotides 800-1100, (iv) nucleotides 900-1200 and (v) nucleotides 1100-1356. These regions in wild-type DNA encompass terminator sequences.
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申请公布号 |
US5571694(A) |
申请公布日期 |
1996.11.05 |
申请号 |
US19940280228 |
申请日期 |
1994.07.25 |
申请人 |
EVANS MEDICAL LIMITED |
发明人 |
MAKOFF, ANDREW J.;ROMANOS, MICHAEL A.;CLARE, JEFFREY J.;FAIRWEATHER, NEIL F. |
分类号 |
C12N1/19;A61K38/00;A61K39/08;A61P31/04;C07K14/005;C07K14/195;C07K14/33;C07K14/41;C12N15/31;C12N15/81;C12P21/00;C12P21/02;C12R1/145;C12R1/865;(IPC1-7):A61K39/08;C12N15/37 |
主分类号 |
C12N1/19 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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