Transcription and nucleic acid sequence determination with short primer DNA/RNA molecules and RNA polymerase

摘要

In vitro transcription of RNA or DNA templates from short RNA or DNA primers is acheived in the absence of a DNA promoter sequence. The progressive nature of the elongation complex according to the present invention allows for increased yields of fully extended transcripts and minimizes aborted RNA chains normally associated with in vitro transcription initiation at a promoter.