| 摘要 |
PURPOSE: To efficiently obtain an acetylisoflavone glycoside having pharmacological actions such as estrogen action, antimicrobial action, anti-oxidant action and anticancer action by carrying out decarboxylation reaction of malonylisoflavohe glycoside extracted from soybean in an aprotic solvent. CONSTITUTION: Soybean is heated so that the temperature of the soybean becomes about 80 deg.C under atmosphere of >=120 deg.C and divided into half by a rubber roller and cooled and seed coat is separated and removed from the soybean and the seed coat-free soybean is immersed into a warm water kept to 50 deg.C for 2hr while controlling pH to 8.0 and the resultant extracted solution is brought into contact with a synthetic absorbing agent to absorb malonylisoflavone glycoside in the extracted solution. The absorbed material is eluted using an aqueous solution of an alcohol to afford malonylisoflavone glycoside. Then, the malonylisoflavone glycoside is dissolved in an aprotic solvent such as dimethylformamide and allowed to stand. Thereby, the glycoside is subjected to decarboxylation reaction to efficiently provide the objective acetylisoflavone glycoside such as acetyldaizin of formula I or acetylgenistin of formula II. |
