| 摘要 |
<p>The present invention provides an efficient method for the C-terminal sequencing of proteins or peptides under acidic conditions using acetyl chloride or phosphoryl chloride reacting with a suitable isothiocyanate for derivatization of the carboxy terminus to a thiohydantoin amino acid derivative. Cleavage of the derivatized thiohydantoin amino acid occurs with thiocyanic acid and acetic acid in water and also with the use of a buffer and potassium or sodium thiocyanate or potassium or sodium dithionite reagent. The invention also provides C-terminal sequencing of proteins or peptides by first reacting the peptide or protein with an acid chloride reagent, such as acetyl chloride and with an organic isothiocyanate, an organic salt thiocyanate, or a metal thiocyanate to yield a thiohydantoin amino acid derivative. The thiohydantoin amino acid derivative may be cleaved using either acidic or basic conditions.</p> |
