METHOD FOR MEASURING HORMONES IN BIOLOGICAL SAMPLES

摘要

A gene construct, cell line, and method useful for measuring hormones, preferably 1,25-dihydroxyvitamin D3 and vitamin D analogs is disclosed. The gene construct comprises a promoter operably connected to a reporter gene, wherein the promoter contains a hormone response element. The cell line is permanently transformed with the construct. The method comprises exposing a sample of the substance containing the hormone to the cell line, measuring the expression level of the reporter gene and correlating the expression level with hormone concentration.