| 摘要 |
A fluorescence spectral overlap electronic compensation circuit for high speed flow cytometry systems is provided. In high speed flow cytometry systems, such as systems with a pulse rise time up to 2 mu s, baseline restoration circuits may not adequately eliminate the DC offset of input signals, in which case, DC offset will result in errors after overlap compensation. In addition, analog spectral overlap compensation operations may result in signal distortions that are unacceptable when using a log amp whose output signal depends on the absolute value of its input signal. The disclosed fluorescence spectral overlap compensation circuit includes an adjustable DC offset compensation circuit to more accurately reduce the DC offset components of signals, and a half-wave rectifier for eliminating the signal distortions caused by spectral overlap compensation operations. |
