LYTIC SYSTEM UTILIZING PROPIONIC ACID FOR LEUKOCYTES DIFFERENTIATION

摘要

A lytic reagent system for use in the differentiation of leukocyte sub-populations is provided. The lytic reagent system includes a diluent, a lytic reagent consisting essentially of propionic acid at a concentration sufficient to cause lysis of the erythrocyte fraction of the blood sample but leave substantially intact the leukocyte fraction for subsequent differentiation, a detergent at a concentration sufficient to promote clarification and proper sizing of the leukocyte sub-populations, and a quench comprising a mixture of salt solution for neutralizing the system.